ANALISIS TOTAL COLIFORM DAN ESCHERICHIA COLI DENGAN METODE COLILERT- 18 PADA SAMPEL AIR SUMUR
Keywords:
Well Water, Colilert-18, Total Coliform, Escherichia Coli, Water Quality, MPN.Abstract
Water is an essential necessity for human life and must meet quality standards, particularly in terms of microbiological aspects. This study aims to analyze the presence of total coliform bacteria and Escherichia coli in well water samples using the Colilert-18 method. The testing was conducted at the UPTD Environmental Laboratory of the Environmental and Forestry Service (DLHP) of South Sumatra Province on five well water samples. The Colilert-18 method was chosen due to its advantages in rapidly and accurately detecting bacteria through enzymatic reactions indicated by color changes and fluorescence. The results showed that all well water samples tested positive for total coliform, with values ranging from 195.6 to >2419.6 MPN/100 mL. Meanwhile, Escherichia coli was detected in four samples with values ranging from 4.1 to 770.1 MPN/100 mL, while one sample showed no detection. The high levels of bacterial contamination in most samples indicate microbiological pollution, which is likely caused by fecal contamination due to poor environmental sanitation conditions. Based on these findings, it can be concluded that most well water samples do not meet the hygiene and sanitation water quality standards according to applicable regulations. Therefore, water treatment efforts and improvements in environmental sanitation are necessary to reduce contamination risks and their impacts on public health.
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