KELIMPAHAN BAKTERI PADA SALURAN PENCERNAAN (USUS DAN HEPATOPANKREAS) UDANG VANAME (LITOPENAEUS VANNAMEI) HASIL BUDI DAYA MENGGUNAKAN ANGKA LEMPENG TOTAL
Keywords:
Digestive Tract Bacteria, Litopenaeus Vannamei, Total Plate Count.Abstract
Shrimp is one of Indonesia's leading aquaculture commodities with increasing export demand, making product quality improvement a critical priority. The digestive tract of white leg shrimp, particularly the intestine and hepatopancreas, serves as the primary habitat for diverse microbial communities that play significant roles in nutrient metabolism and shrimp growth. This study aimed to determine the abundance of bacteria in the digestive tract of cultured white leg shrimp using the Total Plate Count method. Samples were collected from shrimp raised at the Fish Health and Environment Testing Center (BPKIL) Serang, with three shrimp taken from each of six containers coded K1–K3 and E1–E3. The intestine and hepatopancreas were dissected, weighed, and processed through serial dilutions from 10⁻¹ to 10⁻⁵, then spread onto Plate Count Agar and incubated at 35°C for 24 hours following SNI 2332.3:2015 procedures. Observations were conducted on January 14, 16, 18, and 21, 2026. The results showed that bacterial abundance in the digestive tract ranged from 10⁶ CFU/mL throughout the observation period, with the highest average values recorded on January 18, 2026, reaching 3.05 × 10⁶ CFU/mL for intestine samples and 2.97 × 10⁶ CFU/mL for hepatopancreas samples. These findings indicate that the digestive tract of white leg shrimp constitutes a highly supportive environment for microbial growth, influenced by nutrient availability from feed digestion and prevailing culture conditions.
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References
Ahmad, M.A., T. Harijono, dan K. Primasari. "Teknik Produksi Naupli Udang Vaname (Litopenaeus vannamei) di Hatchery CV. Pacific Andre Anton (PAA) Kabupaten Situbondo Provinsi Jawa Timur." Jurnal Riset Rumpun Ilmu Hewani 4, no. 1 (2025): 73–96.
Amin, M., R.R.C. Kumala, A.T. Mukti, M. Lamid, dan D.D. Nindarwi. "Metagenomic Profiles of Core and Signature Bacteria in The Guts of White Shrimp, Litopenaeus vannamei, With Different Growth Rates." Aquaculture 550 (2024): 737849.
Azis, A. Jabarsyah, H. Iromo, dan A. Sucipto. Pembesaran Udang Vanamei Berbasis Bioflok: Peluang dan Strategis Praktis. Jawa Timur: Kramantara Jaya Sentosa, 2025.
Badan Standardisasi Nasional (BSN). SNI 2332.3:2015 Cara Uji Mikrobiologi – Bagian 3: Penentuan Angka Lempeng Total (ALT) pada Produk Perikanan. Jakarta: BSN, 2015.
Blancheton, J. P., K. J. K. Attramadal, L. Michaud, E. R. d'Orbcastel, dan O. Vadstein. "Insight into Bacterial Population in Aquaculture Systems and Its Implication." Aquacultural Engineering 53 (2013): 30–39.
Cornejo-Granados, F., et al. "Host Genome Drives The Microbiota Enrichment of Beneficial Microbes in Shrimp: Exploring The Hologenome Perspective." Animal Microbiome, 2025.
Farraosi, A., E. Saraswati, dan M. Yuniartik. "Analisis Hubungan Bahan Organik dengan Total Kelimpahan Bakteri di Tambak Udang Vaname (Litopenaeus vannamei) Hijau Makmur Desa Pakis Kecamatan Banyuwangi." Journal of Fisheries Sustainability 2, no. 1 (2022): 24–33.
Fithriyani, D., K. Nisa, dan A. Wahyuningtyas. "Analisis Angka Lempeng Total, dan Salmonella sp. pada Minuman Kopi Gula Aren Waralaba." Jurnal Kolaboratif Sains 8, no. 10 (2025): 6085–6092.
Huang, Z., H. Jin, X. Tang, dan J. He. "A Review: Microbes and Their Effect on Growth Performance of Litopenaeus vannamei (White Leg Shrimps) during Culture in Biofloc Technology System." PMC, MDPI, 2024.
Li, E., C. Xu, X. Wang, S. Wang, Q. Zhao, M. Zhang, J. Qin, dan L. Chen. "Gut Microbiota and Its Modulation for Healthy Farming of Pacific White Shrimp (Litopenaeus vannamei)." Reviews in Fisheries Science & Aquaculture 26, no. 3 (2018): 381–399.
Li, M., A. Ghonimy, D.Q. Chen, J.T. Li, Y.Y. He, L.S. López Greco, F. Dyzenchauz, dan Z.Q. Chang. "The Intestine Microbiota of Shrimp and Its Impact on Cultivation." Applied Microbiology and Biotechnology, 2024.
Madigan, M. T., K. Bender, D. Buckley, W. Sattley, dan D. Stahl. Brock Biology of Microorganisms. 15th ed. Pearson, 2018.
Mulianto, D., Widyowati, H. Raharja, dan A. Zubaidah. "Aktivitas Proteolitik Bakteri Kandidat Probiotik Dari Saluran Pencernaan Udang Jerbung (Penaeus merguiensis)." Jurnal Riset Akuakultur 16, no. 4 (2021): 221–229.
Tzuc, J.T., D.R. Escalante, R. Rojas Herrera, G. Gaxiola Cortés, dan M.L.A. Ortiz. "Microbiota from Litopenaeus vannamei: Digestive Tract Microbial Community of Pacific White Shrimp." SpringerPlus 3 (2014): 280.
Ramadani, F. M., S. Salsabila, M. Ratna, S. A. Iskandar, N. R. Hajirah, A. S. Azani, dan E. N. Putri. Teknik Budidaya Udang Vaname Skala Super Intensif. Sulawesi Selatan: Perpustakaan Universitas Negeri Makassar, 2023.
Rizki, Z., Fitriana, dan A. Jumadewi. "Identifikasi Jumlah Angka Kuman pada Dispenser Metode TPC (Total Plate Count)." Jurnal SAGO: Gizi dan Kesehatan 4, no. 1 (2022): 38–43.
Scabra, A. R., M. Marzuki, dan B. M. Yarni. "Pengaruh Pemberian Kalsium Hidroksida (CAOH2) dan Fosfor (P) terhadap Pertumbuhan Udang Vanname (Litopenaeus vannamei) pada Media Air Tawar." Jurnal Ruaya: Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan 11, no. 1 (2023): 39–51.
Suharman, K. N. Izzati, dan N. A. T. Himelda. "Analisis Cemaran Mikroba dalam Produk Minuman Sari Kedelai dengan Metode Total Plate Count (TPC)." Journal of Innovative Food Technology and Agricultural Product 1, no. 1 (2023): 9–13.
Tambunan, E.J., A. Rahmawati, H. Djamaludin, M. Dailami, dan S. Anitasari. Udang Vaname: Dari Hulu ke Hilir. Malang: Universitas Brawijaya Press, 2022.
Zeng, S., Z. Huang, D. Hou, J. Liu, S. Weng, dan J. He. "Composition, Diversity and Function of Intestinal Microbiota in Pacific White Shrimp (Litopenaeus vannamei) at Different Culture Stages." PeerJ 5 (2017): e3986.
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