ANALISIS KANDUNGAN TOTAL COLIFORM DAN ESCHERICHIA COLI PADA AIR BERSIH MENGGUNAKAN METODE MEMBRANE FILTER
Keywords:
Total Coliform, Clean Water, Membrane Filter, Escherichia Coli, Water Quality.Abstract
Clean water quality is a major concern due to the potential contamination by microorganisms, particularly coliform bacteria that can pose health risks to humans. The presence of total coliform and Escherichia coli is commonly used as an indicator of biological contamination in water. This study aims to analyze the total coliform content in clean water using the membrane filter method at the UPTD Environmental Laboratory of the Environmental Service of Lampung Province. The method involved filtering 100 mL of water samples through a sterile membrane, followed by incubation on Chromocult Coliform Agar (CCA) at 37°C for 24 hours, and colony counting using a colony counter. The results showed that all 10 samples had 0 CFU/100 mL for both total coliform and Escherichia coli, indicating no microbiological contamination. These findings confirm that the water quality meets the standards set by the Indonesian Ministry of Health Regulation Number 2 of 2023. In conclusion, the membrane filter method is effective for detecting bacterial indicators, and the tested water is safe for consumption, hygiene, and sanitation purposes.
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Abu-Sini, M. K., Maharmah, R. A., Abulebdah, D. H., dan Al-Sabi, M. N. 2023. Isolation and Identification of Coliform Bacteria and Multidrug-Resistant Escherichia coli from Water Intended for Drug Compounding in Community Pharmacies in Jordan. Healthcare. 11(3): 299.
Atlas, R. M., and Snyder, J. W. 2014. Handbook of Medical for Clinical and Public Health Microbiology. Francis: CRC Press.
Azkhiyati, L., Herawati, D., Santoso, S. D., Pratiwi, E. R., dan Suryani, E. M. 2023. Perbandingan Metode Membran Filter dan Metode Tabung Ganda terhadap Kandungan Escherichia coli pada Air Bersih. Jurnal Sainhealth. 7(1).
Brooks, G., Carroll, K. C., Butel, J., and Morse, S. 2013. Jawetz Melnick dan Adelbergs Medical Microbiology. Edisi ke-26. New York: The McGraw- Hill Companies.
Devika, M. M., Bhuvaneshwari, M. G., dan Mohanram, K. 2019. Comparative Study of Membrane Filtration and Spread Plate Technique for Dialysis Water Analysis. Saudi Journal of Pathology Microbiology. 4(9): 649-653.
Djana, M. 2023. Analisis Kualitas Air Dalam Pemenuhan Kebutuhan Air Bersih di Kecamatan Natar Hajimena Lampung Selatan. Jurnal Redoks. 8(1): 81-87.
Farnandez, L., and Turner, M. 2017. The chronicles of incision management: clinical insights, perspectives, and treatment approaches. Duke University Medical Center and University of Texas Medical Center. 1(1): 1-36.
Febriawati, L., Mellaty, R., dan Widowati, T. 2021. Analisis Aksesibilitas Air Bersih dalam Rangka Peningkatan Ketahanan Keluarga di DKI Jakarta. Jurnal Lemhannas RI. 9(2): 24-39.
Fitrilawati, Fauziah, Y., Maulida, G., dan Syakir, N. 2022. Uji filtrasi model polutan methylen blue menggunakan membran kulit telur. Jurnal Ilmu dan Inovasi Fisika. 6(1): 81-89.
Haq, H. S., Suswati, E., Nurdian, Y., Mufida, D. C., dan Raharjo, A. Μ. 2023. Contamination of Coliform Bacteria on Ocimum basilicum at Traditional Markets, Jember Regency. Saintika Medika. 19(2): 110-118.
Herlina, A., Nugraheni, I. A., Sutopo, M. N., dan Anindita, N. S. 2023. Deteksi bakteri coliform dan Escherichia coli menggunakan metode penyaringan membran filter pada uji sampel air minum konsumen. Prosiding Seminar Nasional Penelitian dan Pengabdian Kepada Masyarakat LPPM Universitas Aisyiyah Yogyakarta. 1: 504-510.
Isnikarita, R., dan Zulfah, N. 2025. Analisis Bakteri Total Coliform dan Escherichia coli pada Air Bersih di Lingkungan Universitas Islam Indonesia Menggunakan Media Chromogenic Coliform Agar. Jurnal Serambi Engineering. 10(1).
Jiang, X., Morgan, J., and Doyle, M. P. 2002. Fate of Escherichia coli O157:H7 in manure-amended soil. Applied and Environmental Microbiology. 68(5): 2605-2609.
Lim, J. Y., Yoon, J. W., dan Hovde, C. J. 2010. A brief overview of Escherichia coli O157:H7 and its plasmid O157. Journal of Microbiology and Biotechnology. 20(1):5.
Matlubah, U. S., Fitrianarni, D., Alam, Y. B., Firdhausi, N., dan Faizah, H. 2024. Uji bakteri coliform dan Escherichia coli pada sampel air minum menggunakan metode filtrasi dan total plate count (tpc). Jurnal Kesehatan Ilmiah Indonesia. 9(2): 1-7.
Ningrum, S. O. 2018. Analisis kualitas badan air dan kualitas air sumur di sekitar pabrik gula Rejo Agung Baru Kota Madiun. Jurnal Kesehatan Lingkungan. 10(1): 1-12.
Pal, P. 2014. Detection of coliforms in drinking water and its effect on human health - A review. International Letters of Natural Sciences. 12(2).
Permenkes. 2023. Peraturan Menteri Kesehatan RI Nomor 2/MENKES/2023 tentang Peraturan Pelaksanaan Peraturan Pemerintah Nomor 66 Tahun 2014 tentang Kesehatan Lingkungan.
Rifai, K. R. 2021. Uji indole sebagai kegiatan penjaminan mutu tambahan pada hasil pengujian coliform dalam sampel air mineral. Jurnal Teknologi Proses dan Inovasi Industri. 6(1): 1-6.
Rizaldi, A., Zelpina, E., dan Oktarina, K. 2022. Cemaran coliform dan total plate count pada daging ayam broiler: studi kasus di pasar tradisional kabupaten barito timur. Jurnal Sains dan Teknologi Peternakan. 4(1): 28-33.
Rizki, Z., Fitriana, dan Jumadewi, A. 2022. Identifikasi jumlah angka kuman pada dispenser metode tpc (total plate count). Sago: Gizi dan Kesehatan. 4(1): 38-43.
Seo, M., Lee, H., and Kim, Y. 2019. Relationship between coliform bacteria and water quality factors at weir stations in the Nakdong River, South Korea. Water. 11(6): 1171.
Suharyono. 2008. Diare Akut Klinik dan Laboratorik. Jakarta: Rhineka Cipta.
Suriawira, U. 1996. Air Dalam Kehidupan dan Lingkungan yang Sehat. Bandung: Penerbit Alumni.
Suriawira, U. 1996. Air Dalam Kehidupan dan Lingkungan yang Sehat. Bandung: Penerbit Alumni.
Syamsussabri, M., Ningtyas, R. N., Najah, A. A., Fahmi, M. S., dan Suarsini, E. 2018. Analysis of coliform bacteria contamination in drinking water sources in Malang City. El-Hayah: Jurnal Biologi. 7(1): 28-35.
Syuhada, F. A., Pulungan, A. N., Sutiani, A., Nasution, H. I., Sihombing, J. L., dan Herlinawati, H. 2021. Pengabdian Kepada Masyarakat (PKM) dalam Pengolahan Air Bersih di Desa Sukajadi. Jurnal Pengabdian Kepada Masyarakat (JPKM) TABIKPUN. 2(1): 1-10.
Wahyuningsih, E. S., Gunarti, N. S., Fikayuniar, L., dan Fajriyani, A. 2023. Uji Organoleptik dan Mikrobiologi Air Minum Isi Ulang di Sekitar UBP Karawang. Media Bina Ilmiah. 17(9): 2199-2206.
Widyaningsih, W., Supriharyono, S., dan Widyorini, N. 2016. Analisis total bakteri coliform di perairan muara Kali Wiso Jepara. Management of Aquatic Resources Journal (MAQUARES). 5(3): 157-164.
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